Beilstein J. Org. Chem.2015,11, 1741–1748, doi:10.3762/bjoc.11.190
utilization pathway of Pichia pastoris for improved NADH regeneration. By deleting the genes coding for dihydroxyacetonesynthase isoform 1 and 2 (DAS1 and DAS2), NADH regeneration via methanol oxidation (dissimilation) was increased significantly. The resulting Δdas1 Δdas2 strain performed better in
an efficient and easy-to-use strategy for NADH-dependent whole-cell conversions. At the same time methanol serves as co-solvent, inductor for catalyst and cofactor regeneration pathway expression and source of energy.
Keywords: bioreduction; cofactor regeneration; dihydroxyacetonesynthase; methanol
generated via the dissimilatory pathway and full oxidation of the single carbon molecule methanol. Alternatively, in a simplified model formaldehyde is condensed with D-xylulose-5-phosphate and subsequently converted into dihydroxyacetone and D-glyceraldehyde-3-phosphate by dihydroxyacetonesynthase (DAS
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Graphical Abstract
Figure 1:
Simplified schematic representation of the methanol utilization pathway in Pichia pastoris. The mai...